DDIT3 抗体 (pSer30)
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北京 101111
Quick Overview for DDIT3 抗体 (pSer30) (ABIN482168)
抗原
See all DDIT3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 15-64, pSer30
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原理
- CHOP (phospho Ser30) Antibody
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特异性
- Phospho-CHOP (S30) Polyclonal Antibody detects endogenous levels of CHOP protein only when phosphorylated at S30.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human CHOP around the phosphorylation site of Ser30. AA range:15-64
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亚型
- IgG
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))
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别名
- CHOP
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背景
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Synonyms: DDIT3, CHOP, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, DDIT-3, C/EBP-homologous protein, CHOP, C/EBP-homologous protein 10, CHOP-10, Growth arrest and DNA damage-inducible protein GADD153
Background: This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010],
Gene Name: DDIT3
Protein Name: DNA damage-inducible transcript 3 protein
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分子量
- 19 kDa
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基因ID
- 1649
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OMIM
- 126337
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UniProt
- P35638
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途径
- Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis
抗原
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