EIF4B 抗体 (pSer422)
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Quick Overview for EIF4B 抗体 (pSer422) (ABIN1531431)
抗原
See all EIF4B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 388-437, pSer422
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原理
- eIF4B (phospho Ser422) Antibody
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特异性
- Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422. AA range:388-437
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亚型
- IgG
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))
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别名
- eIF4B
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背景
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Synonyms: EIF4B, Eukaryotic translation initiation factor 4B, eIF-4B
Background: function:Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.,
Gene Name: EIF4B
Protein Name: Eukaryotic translation initiation factor 4B
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分子量
- 69 kDa
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基因ID
- 3716
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OMIM
- 147795
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UniProt
- P23588
抗原
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