TNNI3 抗体 (pSer22, pSer23)
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Quick Overview for TNNI3 抗体 (pSer22, pSer23) (ABIN1531404)
抗原
See all TNNI3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 5-54, pSer22, pSer23
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原理
- Troponin I-C (phospho Ser22/S23) Antibody
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特异性
- Phospho-Troponin I-C (S22/S23) Polyclonal Antibody detects endogenous levels of Troponin I-C protein only when phosphorylated at S22/S23.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from mouse TNNI3 around the phosphorylation site of Ser22 and Ser23. AA range:5-54
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亚型
- IgG
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- TNNI3 (Cardiac Troponin I (TNNI3))
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别名
- TNNI3
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背景
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Synonyms: TNNI3, TNNC1, Troponin I, cardiac muscle, Cardiac troponin I
Background: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac.This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
Gene Name: Tnni3
Protein Name: Troponin I cardiac muscle
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分子量
- 24 kDa
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UniProt
- P19429
抗原
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