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Splicing Factor 1 抗体 (pSer82)

The 兔 多克隆 anti-Splicing Factor 1 antibody (ABIN1531391) specifically detects Splicing Factor 1 in WB, IHC, ELISA 和 IF. The antibody is reactive with 人, 小鼠 和 猴 samples.
产品编号 ABIN1531391
发货至: 中国
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Quick Overview for Splicing Factor 1 抗体 (pSer82) (ABIN1531391)

抗原

See all Splicing Factor 1 (SF1) 抗体
Splicing Factor 1 (SF1)

适用

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人, 小鼠, 猴

宿主

  • 97
  • 31
兔

克隆类型

  • 93
  • 35
多克隆

标记

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This Splicing Factor 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • 抗原表位

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    AA 48-97, pSer82

    原理

    Splicing factor 1 (phospho Ser82) Antibody

    特异性

    Phospho-Splicing factor 1 (S82) Polyclonal Antibody detects endogenous levels of Splicing factor 1 protein only when phosphorylated at S82.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    纯度

    > 95 %

    免疫原

    The antiserum was produced against synthesized peptide derived from human SF1 around the phosphorylation site of Ser82. AA range:48-97

    亚型

    IgG
  • 应用备注

    WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C/1 year

    有效期

    12 months
  • 抗原

    Splicing Factor 1 (SF1)

    别名

    SF1

    背景

    Synonyms: SF1; ZFM1; ZNF162; Splicing factor 1; Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162

    Background: This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016],

    Gene Name: SF1

    Protein Name: Splicing factor 1

    分子量

    68 kDa

    基因ID

    7536

    OMIM

    601516

    UniProt

    Q15637

    途径

    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
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