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EN2 抗体 (AA 281-333) (HRP)

This HRP-conjugated 兔 多克隆 anti-EN2 antibody specifically detects EN2 in WB, ELISA, IHC (p) 和 IHC (fro). The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN1409840
发货至: 中国
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Quick Overview for EN2 抗体 (AA 281-333) (HRP) (ABIN1409840)

抗原

See all EN2 抗体
EN2 (Engrailed Homeobox 2 (EN2))

适用

  • 56
  • 30
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
人, 小鼠

宿主

  • 52
  • 3
  • 1

克隆类型

  • 53
  • 3
多克隆

标记

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  • 1
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  • 1
  • 1
  • 1
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  • 1
  • 1
  • 1
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This EN2 antibody is conjugated to HRP

应用范围

  • 44
  • 23
  • 13
  • 13
  • 8
  • 7
  • 6
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

    • 18
    • 14
    • 9
    • 5
    • 1
    AA 281-333

    交叉反应

    人, 小鼠

    预测反应

    Rat,Dog,Sheep,Pig

    纯化方法

    Purified by Protein A.

    免疫原

    KLH conjugated synthetic peptide derived from human EN2/Engrailed 2

    亚型

    IgG
  • 应用备注

    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 μg/μL

    缓冲液

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    注意事项

    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

    储存条件

    -20 °C

    储存方法

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    EN2 (Engrailed Homeobox 2 (EN2))

    别名

    EN2/Engrailed 2

    背景

    Synonyms: Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2

    Background: The engrailed-2 gene, EN2, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homologs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expression of a-synuclein, a gene that is genetically linked to Parkinson?s disease. During early brain development mouse EN-2 is expressed in a broad band across most of the mid-hindbrain region. EN-2 is also expressed in mouse myoblasts and has been assiciated with cerebellar hypoplasia.

    基因ID

    2020

    UniProt

    P19622
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