Gemin 3/Sma (AA 101-200) 抗体 (AbBy Fluor® 555)
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- 抗原
- Gemin 3/Sma
- 抗原表位
- AA 101-200
- 适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- AbBy Fluor® 555
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应用范围
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- 预测反应
- Human,Mouse,Rat,Dog,Chicken
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human Gemin 3
- 亚型
- IgG
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- 应用备注
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Gemin 3/Sma
- 背景
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Synonyms: Gemin3, Component of gems 3, DDX 20, DDX20, DDX20_HUMAN, DEAD Asp Glu Ala Asp box polypeptide 20, DEAD Asp-Glu-Ala-Asp box polypeptide 20, DEAD box protein 20, DEAD box protein DP 103, DEAD box protein DP103, DEAD-box protein DP103, DEAD/H Asp Glu Ala Asp/His box polypeptide 20 103kD, DEAD/H Asp Glu Ala Asp/His box polypeptide 20, DEAD/H BOX 20, DKFZP434H052, DP 103, DP103, Gemin-3, Gemin3, Probable ATP dependent RNA helicase DDX20, Probable ATP-dependent RNA helicase DDX20, SMN interacting protein.
Background: Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of motor neurons in the spinal cord. SMA is caused by deletion or loss-of-function mutations in the SMN (survival of motor neuron) gene. Gemin3, also known as DP103, DDX20, DEAD-box protein DP130 and DEAD/H box 20, is a protein product of human chromosome 1p13.2. It associates directly with SMN and is a part of the SMN complex containing Gemin2, Gemin4, Gemin5 and Gemin6, as well as several spliceosomal snRNP proteins. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing of the nucleus. It is found in both the cytoplasm and the nucleus. The nuclear form is concentrated in subnuclear bodies called gems (for Gemini of the coiled bodies). Gemin3 also interacts with SmB, SmD2 and SmD3. It contains the conserved motif Asp-Glu-Ala-Asp (DEAD) characteristic of DEAD-box proteins. Gemin3 is a putative RNA helicase and shows ATPase activity. It is expressed in B and T cell neuroblastoma-derived cell lines, malignant melanoma tumor, normal testis and is expressed in low levels in colon, skeletal muscle, liver, kidney and lung.
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