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CD8 alpha 抗体 (AA 51-150)

Name: CD8 alpha 抗体 (AA 51-150)
SKU: ABIN1387948
Availability: OutOfStock

Cited in 2+ publications. This anti-CD8 alpha antibody is a 兔多克隆 antibody detecting CD8 alpha in FACS, WB, ELISA, IHC (fro), IHC (p), IF (p) 和 IF (cc). Suitable for 人, 大鼠和小鸡.

Paraformaldehyde-fixed, paraffin embedded rat spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (CD8 alpha 抗体 (AA 51-150))

IHC (p)

Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8 alpha Polyclonal Antibody, Unconjugated (ABIN1387948) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. (CD8 alpha 抗体 (AA 51-150))

IF (p)

3 images

(2 references)

产品编号 ABIN1387948

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD8 alpha 抗体 (AA 51-150) (ABIN1387948)

抗原

See all CD8 alpha (CD8A) 抗体

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

适用

人, 大鼠, 小鸡

宿主

兔

克隆类型

多克隆

标记

This CD8 alpha antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

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AA 51-150

交叉反应

小鸡, 人, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from rat CD8 alpha

亚型

IgG
anti-CD8a Molecule (CD8A) (AA 22-235) antibody
CD8A 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 8H8A8 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A 适用: 人 IHC, WB, IP, ELISA 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody

anti-CD8a Molecule (CD8A) antibody
CD8A 适用: 人 FACS, WB, IF (p) 宿主: 兔 Monoclonal 23GB1420 unconjugated Recombinant Antibody

anti-CD8a Molecule (CD8A) antibody
CD8A 适用: 小鼠 FACS, IP, ICC, IHC (fro), IHC (p), Func 宿主: 大鼠 Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A 适用: 小鼠 FACS, IP, ICC, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal C8-468 unconjugated

anti-CD8a Molecule (CD8A) (AA 22-182) antibody
CD8A 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 6A9A5 unconjugated

anti-CD8a Molecule (CD8A) (AA 28-196) antibody
CD8A 适用: 小鼠, 大鼠 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-CD8a Molecule (CD8A) (AA 150-180) antibody
CD8A 适用: 人 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-CD8a Molecule (CD8A) (AA 59-88) antibody
CD8A 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Savvatis, Müller, Fröhlich, Pappritz, Zietsch, Hamdani, Grote, Schieffer, Klingel, Van Linthout, Linke, Schultheiss, Tschöpe: "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." in: Basic research in cardiology, Vol. 109, Issue 6, pp. 449, (2014) (PubMed).

Cheng, Guo, Qiao, Liu, Nie, Li, Wang, Jiang: "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." in: Diagnostic pathology, Vol. 8, pp. 168, (2013) (PubMed).
抗原

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

别名

CD8 alpha

背景

Synonyms: T-cell surface glycoprotein CD8 alpha chain, CD8 antigen 32 kDa chain, OX-8 membrane antigen, CD8a
Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

基因ID

24930

UniProt

P07725

途径

TCR Signaling