我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CD8 alpha 抗体 (AA 51-150)

Name: CD8 alpha 抗体 (AA 51-150)
SKU: ABIN1387948
Availability: OutOfStock

This anti-CD8 alpha antibody is a 兔多克隆 antibody detecting CD8 alpha in FACS, WB, ELISA, IHC (fro), IHC (p), IF (p) 和 IF (cc). Suitable for 人, 大鼠和小鸡. This Primary Antibody has been cited in 2+ publications.

Paraformaldehyde-fixed, paraffin embedded rat spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (CD8 alpha 抗体 (AA 51-150))

IHC (p)

Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8 alpha Polyclonal Antibody, Unconjugated (ABIN1387948) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. (CD8 alpha 抗体 (AA 51-150))

IF (p)

3 images

(2 references)

产品编号 ABIN1387948

发货至: 中国

Quick Overview for CD8 alpha 抗体 (AA 51-150) (ABIN1387948)

抗原

See all CD8 alpha (CD8A) 抗体

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

适用

人, 大鼠, 小鸡

宿主

兔

克隆类型

多克隆

标记

This CD8 alpha antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Discover our top product CD8A Primary Antibody

抗原表位
15

13

12

11

9

9

9

9

5

5

4

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 51-150

交叉反应

小鸡, 人, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from rat CD8 alpha

亚型

IgG
anti-CD8a Molecule (CD8A) (AA 22-235) antibody
CD8A 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 8H8A8 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A 适用: 人 IHC, WB, IP, ELISA 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody

anti-CD8a Molecule (CD8A) antibody
CD8A 适用: 小鼠 FACS, IP, IHC (fro), IHC (p), ICC, Func 宿主: 大鼠 Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) antibody (FITC)
CD8A 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 53-6-7 FITC

anti-CD8a Molecule (CD8A) antibody (PE)
CD8A 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 53-6-7 PE

anti-CD8a Molecule (CD8A) antibody
CD8A 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal C8-468 unconjugated

anti-CD8a Molecule (CD8A) antibody
CD8A 适用: 小鼠 FACS, IP, IHC (fro), IHC (p), ICC 宿主: 大鼠 Monoclonal 53-6-7 unconjugated

anti-CD8a Molecule (CD8A) (AA 22-182) antibody
CD8A 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 6A9A5 unconjugated

anti-CD8a Molecule (CD8A) (AA 28-196) antibody
CD8A 适用: 小鼠, 大鼠 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-CD8a Molecule (CD8A) (AA 150-180) antibody
CD8A 适用: 人 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Savvatis, Müller, Fröhlich, Pappritz, Zietsch, Hamdani, Grote, Schieffer, Klingel, Van Linthout, Linke, Schultheiss, Tschöpe: "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." in: Basic research in cardiology, Vol. 109, Issue 6, pp. 449, (2014) (PubMed).

Cheng, Guo, Qiao, Liu, Nie, Li, Wang, Jiang: "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." in: Diagnostic pathology, Vol. 8, pp. 168, (2013) (PubMed).
抗原

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

别名

CD8 alpha

背景

Synonyms: T-cell surface glycoprotein CD8 alpha chain, CD8 antigen 32 kDa chain, OX-8 membrane antigen, CD8a
Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

基因ID

24930

UniProt

P07725

途径

TCR Signaling