Toxin A 抗体
This anti- antibody is a Chicken egg antibody detecting in ELISA 和 WB. Suitable for Clostridium difficile.
Quick Overview for Toxin A 抗体 (ABIN1381735)
抗原
Toxin A
适用
Clostridium difficile
宿主
Chicken egg
标记
非结合性
应用范围
ELISA, Western Blotting (WB)
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产品特性
- Chickens were immunized with highly purified Toxin A purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin A was immunoaffinity purified using the immunogen immobilized on a solid support.
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纯化方法
- egg yolk-derived purified IgY, affinity purified
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anti-Toxin A antibody
适用: Clostridium difficile ELISA, WB 宿主: 小鼠 Monoclonal unconjugated
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应用备注
- This antibody is suitable for ELISA and Western Blotting procedures. By Western blot, a 1:4000 dilution of the IgY was an effective probe for 1 ug of Toxin A. By Direct ELISA, the half-point titre was 1:20,000 using a 2 ug/ml coating of Toxin A. Optimal working dilutions should be determined for your particular assay conditions.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.0 mg/ml
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缓冲液
- Phosphate Buffered Saline (PBS), pH 7.2
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- Toxin A
抗原
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