Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature
Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
样本量
1 mL
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
储存液
Without preservative
注意事项
Each reagent is stable for the period shown on the bottle label if stored as directed.
储存条件
4 °C
储存方法
Store at 2-8°C
抗原
CD8B
(CD8b Molecule (CD8B))
别名
CD8b
背景
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two chains, and (ii) a heterodimer of an chain and a chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8- and CD8-chains, a relatively large proportion of the CD8+ TCR- cells in the spleens of embryos and young chicks express only the -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- CD8 cells in the chicken that is not found in rodents.3 Chicken CD8 is expressed on approximately 80 % of thymocytes, 45 % of blood mononuclear cells and 50 % of spleen cells, but <1 % of cells in the bursa and bone marrow.