CD86 抗体
Quick Overview for CD86 抗体 (ABIN135277)
抗原
See all CD86 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Mouse CD86, Mr 80 kDa
-
产品特性
- Rat Anti-Mouse CD86-LE/AF
-
纯化方法
- Ion exchange column
-
内毒素水平
- Low endotoxin
-
免疫原
- LPS-activated CBA/Ca mouse spleen B cells
-
亚型
- IgG2a
-
-
-
-
应用备注
-
- Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature
- Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY7, AF647, and AF700 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
-
说明
-
In vivo and in vitro blocking assays
-
样本量
- 1 mL
-
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- 0.5 mg of purified immunoglobulin in 1.0 mL of PBS
-
储存液
- Azide free
-
注意事项
- Each reagent is stable for the period shown on the bottle label if stored as directed.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
-
-
- CD86
-
别名
- CD86
-
背景
- CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation. CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.
-
途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
-