Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Adhesion - Reported in literature , Block - Reported in literature
Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PE conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
样本量
1 mL
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
0.5 mg of purified immunoglobulin in 1.0 mL of PBS
储存液
Azide free
注意事项
Each reagent is stable for the period shown on the bottle label if stored as directed.
储存条件
-20 °C
储存方法
Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
CD49d, the 4 chain (100 kDa) of the VLA-4 integrin heterodimer (Mr 150 kDa) VLA-4 belongs to the 1-integrin family, which functions both as cell-matrix and cell-cell receptors.1-3 As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive.