GAPDHS 抗体 (FITC)
Quick Overview for GAPDHS 抗体 (FITC) (ABIN1302845)
抗原
See all GAPDHS 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-GAPDHS FITC
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特异性
- The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
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交叉反应 （详细）
- Human, Porcine
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纯化方法
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
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亚型
- IgM
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-408) antibody
GAPDHS 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E3-2E10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibodyGAPDHS 适用: 人 WB, IHC (p), IHC 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 104-134) antibodyGAPDHS 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB16542 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibodyGAPDHS 适用: Pig WB, IF 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 251-350) antibodyGAPDHS 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-408) antibodyGAPDHS 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibodyGAPDHS 适用: 人, Pig WB, FACS, ICC 宿主: 小鼠 Monoclonal Hs-8 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) antibodyGAPDHS 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 107-156) antibodyGAPDHS 适用: 人, 小鼠, Pig, 猴, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-408) antibodyGAPDHS 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.
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限制
- 仅限研究用
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缓冲液
- Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user. -
储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))
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别名
- GAPDHS
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背景
- Glyceraldehyde-3-phosphate dehydrogenase, spermato,GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,SGAPDH, HSD-35
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基因ID
- 26330
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UniProt
- O14556
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途径
- Regulation of Carbohydrate Metabolic Process
抗原
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