kappa Light Chain 抗体 (APC)
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Quick Overview for kappa Light Chain 抗体 (APC) (ABIN1302657)
抗原
See all kappa Light Chain 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu Ig Kappa Light Chain APC
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特异性
- The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
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交叉反应 (详细)
- Human
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纯化方法
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Human IgG-kappa myeloma protein
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亚型
- IgG1 kappa
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应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
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限制
- 仅限研究用
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溶解方式
- No reconstitution is necessary.
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Do not freeze.
Avoid prolonged exposure to light. -
储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." in: Blood, Vol. 114, Issue 25, pp. 5173-81, (2009) (PubMed).
: "Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for ..." in: Haematologica, Vol. 93, Issue 4, pp. 551-9, (2008) (PubMed).
: "Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis." in: American journal of clinical pathology, Vol. 116, Issue 2, pp. 204-10, (2001) (PubMed).
: "Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8522-6, (1992) (PubMed).
: "Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4150-8, (1987) (PubMed).
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: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." in: Blood, Vol. 114, Issue 25, pp. 5173-81, (2009) (PubMed).
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- kappa Light Chain
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别名
- Ig Kappa Light Chain
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背景
- Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin kappa, Igk
抗原
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