FOXP3 抗体
(2 references)Quick Overview for FOXP3 抗体 (ABIN1302424)
抗原
See all FOXP3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-FoxP3 Purified
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特异性
- The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells.
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交叉反应 （详细）
- Human, Mouse
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Full-length His-tagged recombinant murine FoxP3
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亚型
- IgG1 kappa
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anti-Forkhead Box P3 (FOXP3) (AA 190-412) antibody
FOXP3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) (C-Term) antibodyVerified FOXP3 适用: 小鼠 FACS, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) (AA 297-431) antibodyFOXP3 适用: 人 FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 4F12A2 unconjugated
anti-Forkhead Box P3 (FOXP3) (AA 191-412) antibodyFOXP3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) (C-Term) antibodyVerified FOXP3 适用: 人 WB, FACS, ELISA, IHC, IF, ICC 宿主: 山羊 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) (N-Term) antibodyFOXP3 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 马, 犬, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) (AA 191-412) antibodyFOXP3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D1 unconjugated
anti-Forkhead Box P3 (FOXP3) (AA 1-431) antibodyFOXP3 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box P3 (FOXP3) antibody (APC)FOXP3 适用: 人, 小鼠 ICFC 宿主: 小鼠 Monoclonal 3G3 APC
anti-Forkhead Box P3 (FOXP3) (AA 101-431) antibodyFOXP3 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: Recommended dilution: 2 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 75, Issue 12, pp. 1040-50, (2009) (PubMed).
: "Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 17, pp. 6659-64, (2006) (PubMed).
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: "The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 75, Issue 12, pp. 1040-50, (2009) (PubMed).
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- FOXP3 (Forkhead Box P3 (FOXP3))
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别名
- FoxP3
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背景
- Forkhead box P3,FoxP3 (forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.,Scurfin, JM2, AIID, IPEX, PIDX, XPID, DIETER
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基因ID
- 50943
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UniProt
- Q9BZS1
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途径
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
抗原
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