RNF25 抗体 (C-Term)
Quick Overview for RNF25 抗体 (C-Term) (ABIN129713)
抗原
See all RNF25 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-969
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Supplier
- Rockland
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原理
- RNF25 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human RNF25 protein.
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产品特性
- Synonyms: rabbit anti-Rnf25 antibody, E3 ubiquitin protein ligase RNF25 antibody, Ring finger protein 25 antibody, RING-type E3 ubiquitin transferase Rnf25 antibody, RNF 25, RNF-25
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Ring Finger Protein 25 (RNF25) (AA 1-459) antibody
RNF25 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (AA 1-459) antibodyRNF25 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (AA 352-459) antibodyRNF25 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1D10 unconjugated
anti-Ring Finger Protein 25 (RNF25) (C-Term) antibodyRNF25 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (Middle Region) antibodyRNF25 适用: 人, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (AA 377-439) antibodyRNF25 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (AA 201-250) antibodyRNF25 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, Hamster, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (Middle Region) antibodyRNF25 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, Cow, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 25 (RNF25) (AA 27-241) antibodyRNF25 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 54 kDa in size corresponding to RNF25 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:200 - 1:2,000
Immunoprecipitation Dilution: 1 mg
ELISA Dilution: 1:10,000 - 1:40,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- RNF25 (Ring Finger Protein 25 (RNF25))
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别名
- RNF25
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).
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基因ID
- 64320, 34878787
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UniProt
- Q96BH1
抗原
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