PDCD4 抗体 (C-Term)
(11 references)
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北京 101111
Quick Overview for PDCD4 抗体 (C-Term) (ABIN1043890)
抗原
See all PDCD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-965s
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Supplier
- Rockland
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原理
- Pdcd4 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human Pdcd4 protein.
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产品特性
- Synonyms: rabbit anti-PDCD4 antibody, PDCD-4, PDCD 4, Programmed cell death protein 4, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody, Protein 197/15a
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxyl terminus of human Pdcd4 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Programmed Cell Death 4 (PDCD4) (C-Term) antibody
PDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibodyPDCD4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-100) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibodyPDCD4 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal K4C1 unconjugated
anti-Programmed Cell Death 4 (PDCD4) antibodyPDCD4 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer67) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (N-Term) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibodyPDCD4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) antibodyPDCD4 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 121-149) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB33836 unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:100 - 1:300
Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting, immunoprecipitation and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to Pdcd4 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000 - 1:10,000
ELISA Dilution: 1:10,000 - 1:100,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.06 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling." in: Cell systems, Vol. 4, Issue 1, pp. 73-83.e10, (2019) (PubMed).
: "Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells." in: Cell reports, Vol. 25, Issue 8, pp. 2148-2162.e5, (2018) (PubMed).
: "ERK8 is a novel HuR kinase that regulates tumour suppressor PDCD4 through a miR-21 dependent mechanism." in: Oncotarget, Vol. 7, Issue 2, pp. 1439-50, (2016) (PubMed).
: "MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1." in: Molecular and cellular biology, Vol. 34, Issue 22, pp. 4143-64, (2015) (PubMed).
: "The curcumin analog EF24 targets NF-κB and miRNA-21, and has potent anticancer activity in vitro and in vivo." in: PLoS ONE, Vol. 8, Issue 8, pp. e71130, (2014) (PubMed).
: "CD44 variant 9 expression in primary early gastric cancer as a predictive marker for recurrence." in: British journal of cancer, Vol. 109, Issue 2, pp. 379-86, (2013) (PubMed).
: "The microRNA-21-PDCD4 axis prevents type 1 diabetes by blocking pancreatic beta cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 29, pp. 12030-5, (2011) (PubMed).
: "Pdcd4 repression of lysyl oxidase inhibits hypoxia-induced breast cancer cell invasion." in: Oncogene, Vol. 29, Issue 27, pp. 3921-32, (2010) (PubMed).
: "Elucidating the CXCL12/CXCR4 Signaling Network in Chronic Lymphocytic Leukemia through Phosphoproteomics Analysis." in: PLoS ONE, Vol. 5, Issue 7, pp. e11716, (2010) (PubMed).
: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).
: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, (2005) (PubMed).
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: "Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling." in: Cell systems, Vol. 4, Issue 1, pp. 73-83.e10, (2019) (PubMed).
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- PDCD4 (Programmed Cell Death 4 (PDCD4))
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别名
- PDCD4
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.
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基因ID
- 27250, 21735596
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UniProt
- Q53EL6
抗原
-
