PDCD4 抗体 (pSer457)
(3 references)
Quick Overview for PDCD4 抗体 (pSer457) (ABIN129708)
抗原
See all PDCD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer457
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Supplier Product No.
- 600-401-964
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Supplier
- Rockland
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原理
- Pdcd4 phospho S457 Antibody
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交叉反应 （详细）
- This antibody is specific for human Pdcd4 protein phosphorylated at Ser457.
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产品特性
- Synonyms: rabbit anti-PDCD4 pS457 antibody, PDCD-4, PDCD 4, Programmed cell death protein 4, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody, Protein 197/15a
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Ser457 in the human Pdcd4 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Programmed Cell Death 4 (PDCD4) (C-Term) antibody
PDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-100) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibodyPDCD4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (N-Term) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer67) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) antibodyPDCD4 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) antibodyPDCD4 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 121-149) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB33836 unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibodyPDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9G6 unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-260) antibodyPDCD4 适用: 人 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 21.25 - 2.5 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 52 kDa in size corresponding to Pdcd4 protein is expected in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:400,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.95 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Loss of programmed cell death 4 induces apoptosis by promoting the translation of procaspase-3 mRNA." in: Cell death and differentiation, (2011) (PubMed).
: "Programmed cell death 4 loss increases tumor cell invasion and is regulated by miR-21 in oral squamous cell carcinoma." in: Molecular cancer, Vol. 9, pp. 238, (2011) (PubMed).
: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).
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: "Loss of programmed cell death 4 induces apoptosis by promoting the translation of procaspase-3 mRNA." in: Cell death and differentiation, (2011) (PubMed).
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- PDCD4 (Programmed Cell Death 4 (PDCD4))
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别名
- PDCD4
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.
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基因ID
- 27250, 21735596
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UniProt
- Q53EL6
抗原
-
