EIF3F 抗体 (AA 100-125)
(3 references)
Quick Overview for EIF3F 抗体 (AA 100-125) (ABIN129687)
抗原
See all EIF3F 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 100-125
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Supplier Product No.
- 600-401-934
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Supplier
- Rockland
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原理
- EIF3F Antibody
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交叉反应 （详细）
- This affinity-purified antibody is directed against human eIF3f protein.
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产品特性
- Synonyms: rabbit anti-eIF3F Antibody, eIF-3 epsilon antibody, eIF3 p47 antibody, eIF3-p47 subunit antibody, EIF3S5 antibody, Eukaryotic translation initiation factor 3 subunit 5 antibody, Eukaryotic translation initiation factor 3 subunit F, Deubiquitinating enzyme eIF3f
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human eIF3f protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 1-357) antibody
EIF3F 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) antibodyEIF3F 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 88-357) antibodyEIF3F 适用: 人 IHC, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 88-357) antibodyEIF3F 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 258-357) antibodyEIF3F 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (Internal Region) antibodyEIF3F 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (Internal Region) antibodyEIF3F 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 279-328) antibodyEIF3F 适用: 人, 小鼠, 大鼠, Cow, 猴, 兔, 犬, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (AA 97-146) antibodyEIF3F 适用: 人, 小鼠, 大鼠, Cow, 猴, 兔, 马, 豚鼠, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F) (N-Term) antibodyEIF3F 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:200 - 1:2,000
ELISA Dilution: 1:10,000 - 1:40,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "A DGKζ-FoxO-ubiquitin proteolytic axis controls fiber size during skeletal muscle remodeling." in: Science signaling, Vol. 11, Issue 530, (2018) (PubMed).
: "Glycine restores the anabolic response to leucine in a mouse model of acute inflammation." in: American journal of physiology. Endocrinology and metabolism, Vol. 310, Issue 11, pp. E970-81, (2017) (PubMed).
: "Translational suppression of atrophic regulators by miR-23a integrates resistance to skeletal muscle atrophy." in: The Journal of biological chemistry, (2011) (PubMed).
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: "A DGKζ-FoxO-ubiquitin proteolytic axis controls fiber size during skeletal muscle remodeling." in: Science signaling, Vol. 11, Issue 530, (2018) (PubMed).
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- EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))
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别名
- EIF3F
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背景
- Background: eIF3f, also known as eukaryotic translation initiation factor 3 subunit 5, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.
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基因ID
- 8665, 6685511
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UniProt
- O00303
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
-
