APC1 抗体 (pSer377)
Quick Overview for APC1 抗体 (pSer377) (ABIN129650)
抗原
See all APC1 (ANAPC1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer377
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Supplier Product No.
- 600-401-877
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Supplier
- Rockland
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原理
- APC1 phospho S377 Antibody
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交叉反应 （详细）
- This antibody is specific for phosphorylated human APC1 protein at the pS377 residue.
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产品特性
- Synonyms: rabbit anti-APC1 pS377 Antibody, APC-1, APC 1, ANAPC1 antibody, Anaphase promoting complex subunit 1 antibody, Cyclosome subunit 1 antibody, MCPR antibody, Meiotic checkpoint regulator antibody
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纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pS377 of Human Apc1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Anaphase Promoting Complex Subunit 1 (ANAPC1) (AA 12-155) antibody
ANAPC1 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7G9B3 unconjugated
anti-Anaphase Promoting Complex Subunit 1 (ANAPC1) (AA 12-155) antibodyANAPC1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 7G9C7 unconjugated
anti-Anaphase Promoting Complex Subunit 1 (ANAPC1) (AA 1-185) antibodyANAPC1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Anaphase Promoting Complex Subunit 1 (ANAPC1) (pSer688) antibodyANAPC1 适用: 人, 小鼠 IHC, WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Anaphase Promoting Complex Subunit 1 (ANAPC1) (pSer377) antibodyANAPC1 适用: 大鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Anaphase Promoting Complex Subunit 1 (ANAPC1) (Center) antibodyANAPC1 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Anaphase Promoting Complex Subunit 1 (ANAPC1) (AA 654-703), (pSer688) antibodyANAPC1 适用: 人, 小鼠, 大鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 5.0 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 215 kDa in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:200 - 1:1,000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.21 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))
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别名
- ANAPC1
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背景
- Background: APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.
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基因ID
- 64682, 12056971
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UniProt
- Q9H1A4
抗原
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