CDC16 抗体 (pThr580)
Quick Overview for CDC16 抗体 (pThr580) (ABIN129649)
抗原
See all CDC16 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr580
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Supplier Product No.
- 600-401-876
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Supplier
- Rockland
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原理
- APC6 phospho T580 Antibody
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交叉反应 （详细）
- This antibody is specific for phosphorylated human APC6 protein at the pT580 residue.
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产品特性
- Synonyms: rabbit anti-APC6 pT580 Antibody, APC 6, APC-6, Anaphase promoting complex subunit 6 antibody, CDC16Hs antibody, Cell division cycle 16 homolog antibody, Cell division cycle protein 16 homolog antibody, Cyclosome subunit 6 antibody
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纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pT580 of Human Apc6 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (Internal Region) antibody
CDC16 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (AA 371-620) antibodyCDC16 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (AA 526-575), (pSer560) antibodyCDC16 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (C-Term) antibodyCDC16 适用: 人, Pig, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (AA 371-620) antibodyCDC16 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (C-Term) antibodyCDC16 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) antibodyKD Validated CDC16 适用: 人, 小鼠, 大鼠 WB, IF, ICC 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 72 kDa, 71.5 kDa or 65.9 kDa in size corresponding to isoforms of APC6 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:1,000 - 1:10,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.97 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))
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别名
- CDC16
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背景
- Background: APC6 (also known as Cell division cycle protein 16 homolog (CDC16Hs), anaphase promoting complex subunit 6, and cyclosome subunit 6) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is composed of at least 11 subunits. APC6 interacts with PPP5C and CDC20. This protein colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle. Splice variants have been described for this protein.
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基因ID
- 8881, 37537763
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UniProt
- Q13042
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途径
- Regulation of Actin Filament Polymerization
抗原
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