CDC27 抗体 (AA 415-445)
Quick Overview for CDC27 抗体 (AA 415-445) (ABIN129644)
抗原
See all CDC27 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 415-445
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Supplier Product No.
- 600-401-868
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Supplier
- Rockland
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原理
- CDC27 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human Cdc27 protein.
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产品特性
- Synonyms: rabbit anti-CDC27 Antibody, CDC-27, CDC 27, Anaphase Promoting Complex Subunit 3 antibody, APC 3 antibody, CDC27Hs antibody, Cell Division Cycle 27 antibody, Cell division cycle protein 27 homolog antibody
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids 415-445 of Human cdc27.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 551-830) antibody
CDC27 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) antibodyCDC27 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 5C12 unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 258-431) antibodyCDC27 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 1-830) antibodyCDC27 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 395-444) antibodyCDC27 适用: 人, 大鼠, Cow, 马, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (Center) antibodyCDC27 适用: 人, Cow WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (Middle Region) antibodyCDC27 适用: 人, 大鼠, 犬, Cow, 马, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 814-823), (C-Term) antibodyCDC27 适用: 人, 大鼠, 小鼠, 犬, Cow, 非洲爪蟾 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal AF3-1 unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 92 kDa in size corresponding to Cdc27 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:40,000
IF Microscopy Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
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别名
- CDC27
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背景
- Background: Cdc27 (also known as cell division cycle protein 27 homologue, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. Cdc27 is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Cdc27 has a nuclear localization.
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基因ID
- 996, 167466175
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UniProt
- P30260
抗原
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