Swi6 (AA 314-328) 抗体
This 兔 多克隆 antibody specifically detects Swi6 in WB 和 ELISA. It exhibits reactivity toward Schizosaccharomyces pombe.
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产品编号 ABIN129643
Supplier Product No.: 600-401-867
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Room 801-803
Quick Overview for Swi6 (AA 314-328) 抗体 (ABIN129643)
抗原
Swi6
适用
Schizosaccharomyces pombe
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB), ELISA
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抗原表位
- AA 314-328
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Supplier Product No.
- 600-401-867
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Supplier
- Rockland
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原理
- Swi6 Antibody
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交叉反应 （详细）
- BLAST analysis indicates that cross reactivity with homologues of this protein from other sources is not likely.
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产品特性
- Synonyms: rabbit anti-Swi6 antibody, Chromatin-associated protein swi6, swi-6, swi 6, SPAC664.01c, SPAC824.10c
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纯化方法
- Swi6 Antibody is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This anti-Swi6 Antibody was prepared from whole rabbit antiserum produced by repeated immunizations with a synthetic peptide corresponding aa 314-328 of S.pombe Swi6 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Swi6 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:30,000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Swi6 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Swi6
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背景
- Background: Swi6 is also known as chromatin-associated swi6 protein and repression of silent mating type loci protein. This protein recognizes and binds to histone H3 tails that are methylated at Lys-9, leading to epigenetic repression. Swi6 is also involved in the repression of the silent mating-type loci MAT2 and MAT3 and it may be responsible for the compaction of MAT2/3 into a heterochromatin-like conformation which represses the transcription of these silent cassettes. Swi6 also interacts with the cohesion subunit psc3. Swi6 has a nuclear localization and contains 2 chromo domains.
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基因ID
- 2541633, 19114361
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UniProt
- P40381
抗原
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