CDC27 抗体 (AA 415-445)
Quick Overview for CDC27 抗体 (AA 415-445) (ABIN129642)
抗原
See all CDC27 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 415-445
-
Supplier Product No.
- 600-401-866
-
Supplier
- Rockland
-
原理
- CDC27 phospho S426 Antibody
-
交叉反应 （详细）
- This affinity purified antibody is directed against the phosphorylated form of human CDC27 at the pS426 residue.
-
产品特性
- Synonyms: rabbit anti-CDC27 pS426 Antibody, CDC 27, CDC-27, Cell division cycle protein 27 homolog, H-NUC, APC3, Anaphase-promoting complex subunit 3, ANAPC3
-
纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of Human cdc27 amino acids 415-445 surrounding S426.
Immunogen Type: Conjugated Peptide
-
亚型
- IgG
-
-
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 551-830) antibody
CDC27 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) antibodyCDC27 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 5C12 unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 258-431) antibodyCDC27 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 1-830) antibodyCDC27 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 395-444) antibodyCDC27 适用: 人, 大鼠, Cow, 马, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (Center) antibodyCDC27 适用: 人, Cow WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (Middle Region) antibodyCDC27 适用: 人, 大鼠, 犬, Cow, 马, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 814-823), (C-Term) antibodyCDC27 适用: 人, 大鼠, 小鼠, 犬, Cow, 非洲爪蟾 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal AF3-1 unconjugated
-
-
应用备注
-
Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to CDC27 by western blotting in the appropriate cell lysate or extract. Less than 1 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS426 of CDC27.
Western Blot Dilution: 1:500 - 1:2,500
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:25,000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
-
别名
- CDC27, D0S1430E
-
背景
- Background: Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
-
基因ID
- 996, 167466175
-
UniProt
- P30260
抗原
-
