DIAPH2 抗体 (C-Term)
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- 抗原 See all DIAPH2 抗体
- DIAPH2 (Diaphanous Homolog 2 (DIAPH2))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DIAPH2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Supplier Product No.
- 600-401-851
- Supplier
- Rockland
- 原理
- DIA-2 Antibody
- 交叉反应 (详细)
- This affinity purified antibody is directed against human DIA-2.
- 产品特性
- Synonyms: rabbit anti-DIA-2 Antibody, DIA 2, DIA drome, DIA2, DIAPH2, Diaphanous 2, Diaphanous related formin 2, Diaphorase 2, DRF2, POF, POF2
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product DIAPH2 Primary Antibody
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- 应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kDa in size corresponding to DIA-2 by western blotting in the appropriate cell lysate or extract. Alternate splice variants have been described for this protein. This antibody will only react with DIA-156 (isoform 1 of DIA-2).
Western Blot Dilution: 1:1,000 - 1:6,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:12,000 - 1:60,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.4 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- DIAPH2 (Diaphanous Homolog 2 (DIAPH2))
- 别名
- DIAPH2 (DIAPH2 产品)
- 背景
- Background: DIA-2 (also called DIA drome, Dia2, DIAPH2, Diaphanous 2, Diaphanous related formin 2, Diaphorase 2, DRF2, POF and POF2) may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described. DIA-2 is expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes and can be found from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles. Isoform 1 is referred to as DIA-156, whereas isoform 2 is called DIA-12C.
- 基因ID
- 1730, 5803003
- UniProt
- O60879
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