ATAD5 抗体 (AA 63-76)
Quick Overview for ATAD5 抗体 (AA 63-76) (ABIN129617)
抗原
See all ATAD5 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 63-76
-
Supplier Product No.
- 600-401-688
-
Supplier
- Rockland
-
原理
- ELG-1 Antibody
-
交叉反应 （详细）
- This affinity purified antibody is directed against human Elg1 protein.
-
产品特性
- Synonyms: rabbit anti-ELG-1 antibody, ELG1, ELG 1, FRAG1, ATP (GTP)-binding protein, ATPase Family AAA Domain-Containing Protein 5, Chromosome fragility associated gene 1 protein, ATAD5, C17orf41
-
纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.
Immunogen Type: Conjugated Peptide
-
亚型
- IgG
-
-
anti-ATPase Family, AAA Domain Containing 5 (ATAD5) (Internal Region) antibody
Verified ATAD5 适用: 人 ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-ATPase Family, AAA Domain Containing 5 (ATAD5) (AA 1151-1250) antibodyATAD5 适用: 小鼠 ELISA, WB, ICC, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Elg1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:3,000 - 1:15,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.50 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))
-
别名
- ATAD5
-
背景
- Background: ELG1 (also known as ATP(GTP)-binding protein or Chromosome fragility associated gene 1) is involved in a novel RFC complex that is probably involved in DNA damage and repair by ensuring replication fidelity. This antibody detects a band at about 120 kDa in Hela, A431, Jurkat and HEK193 cells. This corresponds to the band size seen in Kanellis P et al. It remains unclear why the band size detected is much less than the 207 kDa predicted in the protein sequence corresponding to CACC44537.2 (Q96QE3), but as our results correspond to those seen in Kanellis P et al. it is likely that the 120 kDa target is ELG1.
-
基因ID
- 79915, 21615526
-
UniProt
- Q96QE3
抗原
-
