CBX4 抗体 (AA 90-115)
Quick Overview for CBX4 抗体 (AA 90-115) (ABIN129594)
抗原
See all CBX4 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 90-115
-
Supplier Product No.
- 600-401-478
-
Supplier
- Rockland
-
原理
- PC2 Antibody
-
交叉反应 （详细）
- Reactivity occurs against human PC2 protein.
-
产品特性
- Synonyms: rabbit anti-PC2 antibody, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2, CBX4
-
纯化方法
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 90-115 of Human PC2 protein.
Immunogen Type: Conjugated Peptide
-
亚型
- IgG
-
-
anti-Chromobox Homolog 4 (CBX4) (AA 397-514) antibody
CBX4 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 6C5G3 unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 1-160) antibodyCBX4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 401-500) antibodyCBX4 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 401-560) antibodyCBX4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 1-290) antibodyCBX4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (N-Term) antibodyCBX4 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 80-110), (N-Term) antibodyCBX4 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB2931 unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 53-102) antibodyCBX4 适用: 人, 小鼠, 犬, Cow, 豚鼠, 兔, Pig, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 150-200) antibodyCBX4 适用: 人, 小鼠, 犬, Cow, 豚鼠, 兔, Pig IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chromobox Homolog 4 (CBX4) (AA 410-437), (C-Term) antibodyCBX4 适用: 人 WB 宿主: 兔 Polyclonal RB2934 unconjugated
-
-
应用备注
-
Application Note: Anti-PC2 antibody has been tested for use in ELISA, WB, and IF. This affinity purified antibody is useful in immunofluorescence staining of cultured cells. In immunofluorescence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. This affinity purified antibody is useful in western blotting using transfected cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure). It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein. Reactivity in other immunoassays is unknown.
Western Blot Dilution: 1:1,000 - 1:4,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:10,000 - 1:40,000
IF Microscopy Dilution: 1:100 - 1:400
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.36 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- CBX4 (Chromobox Homolog 4 (CBX4))
-
别名
- CBX4
-
背景
- Background: PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to depression of proto-oncogene transcription and subsequently to cellular transformation. Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.
-
基因ID
- 8535
-
UniProt
- O00257
抗原
-
