Cyclin B1 抗体 (pSer126)
(1 reference)
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Quick Overview for Cyclin B1 抗体 (pSer126) (ABIN129589)
抗原
See all Cyclin B1 (CCNB1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer126
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Supplier Product No.
- 600-401-464
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Supplier
- Rockland
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原理
- Cyclin B1 phospho S126 Antibody
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交叉反应 （详细）
- This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue.
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产品特性
- Synonyms: rabbit anti-Cyclin B1 pS126 Antibody, Cyclin B1, CCNB1, CCNB, G2/mitotic-specific cyclin-B1
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纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 115-140 of Human Cyclin B1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Cyclin B1 (CCNB1) (C-Term) antibody
CCNB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyCCNB1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (C-Term) antibodyCCNB1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyCCNB1 适用: 人, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal CCNB1-1098 unconjugated
anti-Cyclin B1 (CCNB1) (AA 271-433) antibodyCCNB1 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyKD Validated CCNB1 适用: 人 WB, FACS, ICC 宿主: 兔 Monoclonal 23GB1670 unconjugated Recombinant Antibody
anti-Cyclin B1 (CCNB1) (AA 1-433) antibodyCCNB1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyKO Validated CCNB1 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Cyclin B1 (CCNB1) (pSer126) antibodyCCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (Internal Region) antibodyCCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 48 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:100 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.01 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Artemis links ATM to G2/M checkpoint recovery via regulation of Cdk1-cyclin B." in: Molecular and cellular biology, Vol. 27, Issue 7, pp. 2625-35, (2007) (PubMed).
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: "Artemis links ATM to G2/M checkpoint recovery via regulation of Cdk1-cyclin B." in: Molecular and cellular biology, Vol. 27, Issue 7, pp. 2625-35, (2007) (PubMed).
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- Cyclin B1 (CCNB1)
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别名
- CCNB1
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背景
- Background: Cyclin B1 (also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF). Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. S126 phosphorylation, the site of phosphorylation recognized by this antibody, is involved in an auto-phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
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基因ID
- 891
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UniProt
- P14635
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途径
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
抗原
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