LIM Domain Binding 2 Protein 抗体 (AA 100-125)
Quick Overview for LIM Domain Binding 2 Protein 抗体 (AA 100-125) (ABIN129586)
抗原
See all LIM Domain Binding 2 Protein (LDB2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 100-125
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Supplier Product No.
- 600-401-459
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Supplier
- Rockland
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原理
- Ldb2 Antibody
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交叉反应 (详细)
- This affinity purified antibody is directed against mouse LDB2.
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产品特性
- Synonyms: rabbit anti-LDB2 antibody, rabbit anti-LDB2/CLIM1 antibody, rabbit anti-CLIM1 antibody, LDB-2, CLIM-1, LIM domain-binding protein 2, Carboxyl-terminal LIM domain-binding protein 1, LIM domain-binding factor CLIM1, Nuclear LIM interactor
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 5 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB2 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.06 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- LIM Domain Binding 2 Protein (LDB2) (LIM Domain Binding 2 (LDB2))
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别名
- Ldb2
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背景
- Background: LDB2 (LIM homeobox protein cofactor CLIM-1a). It is suggested that LIM domain binding proteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. LDB2 is a close homologue of LDB1. LDBs are homologs of the Drosophila Chip protein. Three members of the ldb gene family (Ldb1, Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95 % , 73 % and 62 % amino acid identity with mouse Ldb1, respectively.
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基因ID
- 16826, 116642898
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UniProt
- O55203
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途径
- Stem Cell Maintenance
抗原
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