Phosphoserine 抗体
(1 reference)
Quick Overview for Phosphoserine 抗体 (ABIN129572)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-261
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Supplier
- Rockland
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原理
- Phosphoserine Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.
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产品特性
- Synonyms: pS antibody, phospho serine AB, PhosphoSerine (pS) reactive antibody, proteinogenic amino acid antibodies, Phosphoserine [pS] polyclonal antibody, PhosphoSerine antibody produced in rabbit
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纯化方法
- Anti-Phosphoserine Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated serine peptide coupled to agarose.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Phosphoserine Antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.
Immunogen Type: Other
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亚型
- IgG
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anti-Phosphoserine antibody
WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphoserine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 7A3 unconjugated
anti-Phosphoserine (phosphorylated) antibodyWB 宿主: 小鼠 Monoclonal 5B12 unconjugated
anti-Phosphoserine antibodyWB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoserine antibody (FITC)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphoserine antibody (Biotin)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphoserine antibody (Atto 488)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphoserine antibody (Atto 594)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-Phosphoserine antibody (Atto 390)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 390
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Phosphoserine Antibody is tested for use in ELISA, western blotting, Immunohistochemistry-P, and immunoprecipitation assays. The antibody reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues. The antibody reacts with free phosphoserine, phosphoserine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA and flow cytometry.
Western Blot Dilution: 1:500-1:1,000
Immunoprecipitation Dilution: 10 μg/mg protein sample
ELISA Dilution: 1:1,000-1:2,500
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "The serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell-ECM signaling." in: The Journal of cell biology, Vol. 192, Issue 3, pp. 525-40, (2011) (PubMed).
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: "The serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell-ECM signaling." in: The Journal of cell biology, Vol. 192, Issue 3, pp. 525-40, (2011) (PubMed).
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- Phosphoserine
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物质类
- Amino Acid
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背景
- Background: Anti-Phosphoserine Antibody detects phosphorylated Serines. Serine is one of the proteinogenic amino acids. By virtue of the hydroxyl group, serine is classified as a polar amino acid. It is not essential to the human diet, since it is synthesized in the body from other metabolites, including glycine. Serine is important in metabolism in that it participates in the biosynthesis of purines and pyrimidines. It is the precursor to several amino acids including glycine and cysteine, and tryptophan in bacteria. It is also the precursor to numerous other metabolites, including sphingolipids and folate, which is the principal donor of one-carbon fragments in biosynthesis. Anti-phospho Serine Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Immunology, Cancer research and Signal Transduction research.
抗原
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