NCS1 抗体
Quick Overview for NCS1 抗体 (ABIN129571)
抗原
See all NCS1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-216
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Supplier
- Rockland
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原理
- Frequenin Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
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产品特性
- Synonyms: rabbit anti-FREQUENIN antibody, NCS-1, FLUP, FREQ, Neuronal calcium sensor 1, frequenin homolog antibody, Frequenin-like protein antibody, NCS 1 antibody, NCS1 antibody
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Frequenin (recombinant) coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Frequenin (recombinant from Mouse with extensive post-translational modifications)
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Neuronal Calcium Sensor 1 (NCS1) antibody
NCS1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Neuronal Calcium Sensor 1 (NCS1) (AA 1-190) antibodyNCS1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neuronal Calcium Sensor 1 (NCS1) (AA 1-190) antibodyNCS1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E7 unconjugated
anti-Neuronal Calcium Sensor 1 (NCS1) (N-Term) antibodyNCS1 适用: 人, 大鼠, 小鼠, 斑马鱼, Cow, 小鸡, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Neuronal Calcium Sensor 1 (NCS1) (AA 1-190) antibodyNCS1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neuronal Calcium Sensor 1 (NCS1) (Middle Region) antibodyNCS1 适用: 人, 大鼠, 小鼠, 斑马鱼, Cow, 犬, 豚鼠, 马, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neuronal Calcium Sensor 1 (NCS1) (N-Term) antibodyNCS1 适用: 人, 大鼠, 小鼠, 斑马鱼, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neuronal Calcium Sensor 1 (NCS1) (AA 118-144) antibodyNCS1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Neuronal Calcium Sensor 1 (NCS1) (C-Term) antibodyNCS1 适用: 人, 大鼠, 小鼠, 斑马鱼, Cow, 犬, 豚鼠, 马, 小鸡, 非洲爪蟾, Bat, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Neuronal Calcium Sensor 1 (NCS1) antibodyNCS1 适用: 人, 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This product was assayed by immunoblot and found to be reactive against Frequenin at a dilution of 1:5000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-Frequenin is suitable for the detection by immunoblot of human, mouse and rat Frequenin. Anti-Frequenin has also been tested for use in IF using Hippocampal neurons of 17 day old NMRI mice.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
IF Microscopy Dilution: 1:100-1:2500
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- NCS1 (Neuronal Calcium Sensor 1 (NCS1))
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别名
- Ncs1
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背景
- Background: Frequenin, or Neuronal calcium sensor, is a regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. It directly regulates GRK1 (RHOK), but not GRK2 to GRK5. It can substitute for calmodulin and it stimulates PI4KB kinase activity. It is involved in long-term synaptic plasticity through its interaction with PICK1. Ncs1 may also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
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基因ID
- 14299
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NCBI登录号
- NP_062655
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UniProt
- Q8BNY6
抗原
-
