Biotin 抗体 (PE)
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- 抗原 See all Biotin products
- Biotin
- 适用
- 请咨询
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Biotin antibody is conjugated to PE
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-108-098
- Supplier
- Rockland
- 原理
- Biotin Antibody Phycoerythrin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Phycoerythrin, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
- 产品特性
- Synonyms: goat anti-Biotin Antibody PE Conjugation, Phycoerythrin conjugated goat anti-Biotin Antibody, Vitamin B4 antibody
- 纯化方法
- Anti-Biotin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
- 免疫原
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Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Immunogen Type: Other
- 亚型
- IgG
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anti-Biotin antibody
WB, ELISA, FACS, IHC, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibodyWB, FACS, IHC, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibodyWB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibodyELISA, FACS 宿主: 小鼠 Monoclonal SB58c unconjugated
anti-Biotin antibodyELISA, FACS, IP, CyTOF 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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Flow Cytometry Dilution: 1.0 μg/1,000,000 cells
Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Biotin detects biotin and biocytin by ELISA, Immuno-histo-chemistry (IHC), and the antibody also recognizes biotin conjugated to various immunoglobulins. The antibody may be used in amplification techniques, immunoblotting, in situ nucleic acid hybridization, flow cytometry or Immunofluorescence microscopy.
Western Blot Dilution: 1:50-1:200
ELISA Dilution: 1:50-1:200
IF Microscopy Dilution: 1:50-1:200
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 0.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.05 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
- 有效期
- 6 months
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- 抗原
- Biotin
- Abstract
- Biotin 产品
- 物质类
- Chemical
- 背景
- Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Phycoerythrin Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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