MTBP 抗体 (AA 667-812)
Quick Overview for MTBP 抗体 (AA 667-812) (ABIN129549)
抗原
See all MTBP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 667-812
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Supplier Product No.
- 500-301-875
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Supplier
- Rockland
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原理
- MTBP Antibody
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交叉反应 （详细）
- This antibody is directed against human MTBP protein.
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产品特性
- Synonyms: mouse anti-MTBP antibody, mouse anti-mouse double minute 2 antibody, MDM 2 BP antibody, MDM2 (mouse double minute 2) binding protein 104kD antibody, MDM2 (mouse double minute 2) binding protein antibody, MDM2 Binding Protein antibody, MDM2 BP antibody
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纯化方法
- The product was produced from clarified mouse ascetic fluid.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG1 kappa
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anti-MDM2 Binding Protein (MTBP) (AA 853-881), (C-Term) antibody
MTBP 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20020 unconjugated
anti-MDM2 Binding Protein (MTBP) antibodyMTBP 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-MDM2 Binding Protein (MTBP) (AA 567-724) antibodyMTBP 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MDM2 Binding Protein (MTBP) (AA 23-350) antibodyMTBP 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-MDM2 Binding Protein (MTBP) (AA 122-139) antibodyMTBP 适用: 人 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-MDM2 Binding Protein (MTBP) (Middle Region 2) antibodyMTBP 适用: 人 WB, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-MDM2 Binding Protein (MTBP) (AA 122-139), (Middle Region) antibodyMTBP 适用: 人 WB, EIA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 110 kDa in size corresponding to MTBP by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:200 - 1:2,000
ELISA Dilution: 1:2,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- MTBP (MDM2 Binding Protein (MTBP))
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别名
- MTBP
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背景
- Background: The MDM2 onco-protein is over expressed in human cancer cells and it promotes tumorigenesis by directly blocking the function of the p53 tumor suppressor. Studies also indicate that MDM2 regulates cell proliferation via p53 independent mechanisms. MTBP (Mdm2 [Two] Binding Protein) was identified by a yeast two-hybrid screen as an MDM2 binding protein (1). MTBP has been reported to modulate cell cycle progression by a p53 independent mechanism and suppress MDM2-mediated p53 degradation when ectopically expressed in cells (1,2). The human MTBP gene localizes to 8q24 (3) and MTBP RNA is expressed in a wide range of tissues, with highest being in the thymus, testes and ovaries (1).
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基因ID
- 27085
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NCBI登录号
- NP_071328
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UniProt
- Q96DY7
抗原
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