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- 抗原 See all SAE1 抗体
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SAE1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 200-401-913
- Supplier
- Rockland
- 原理
- SAE1 Antibody
- 交叉反应 （详细）
- This purified antibody is directed against human SUMO Activating Enzyme E1 protein.
- 产品特性
- Synonyms: rabbit anti-Sumo Activating Enzyme E1 Antibody, rabbit anti-SAE1 Antibody, SUMO-activating enzyme subunit 1 antibody, Ubiquitin-like 1-activating enzyme E1A antibody
- 纯化方法
- The product was purified from monospecific antiserum by Protein A chromatography.
- 免疫原
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Immunogen: Anti-SUMO Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product SAE1 Primary Antibody
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anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibody
SAE1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G4-1G5 unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodyKO Validated SAE1 适用: 人 WB, IF, IHC (p), ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (Internal Region) antibodySAE1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibodySAE1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 300-329), (C-Term) antibodySAE1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB2887-RB2888 unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibodySAE1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodySAE1 适用: 人 WB, ELISA, IF 宿主: 兔 Monoclonal 9D8 unconjugated Recombinant Antibody
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodySAE1 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G8 unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodySAE1 适用: 人 ICC, DB 宿主: 小鼠 Monoclonal 2946C4a unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (Center) antibodySAE1 适用: 人, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~60 kDa in size corresponding to SAE1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 5.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
- 别名
- SAE1 (SAE1 产品)
- 背景
- Background: SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) is a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1, also called Aos1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.
- 基因ID
- 10055, 42559897
- UniProt
- Q9UBE0
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