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- 抗原 See all UBA1 抗体
- UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This UBA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 200-401-888
- Supplier
- Rockland
- 原理
- Ubiquitin Activating Enzyme E1 Antibody
- 交叉反应 （详细）
- This antibody is directed against human Ubiquitin Activating Enzyme E1 protein.
- 产品特性
- Synonyms: rabbit anti-Ubiquitin Activating Enzyme E1 Antibody, A1S9 antibody, A1S9 protein antibody, A1S9T and BN75 temperature sensitivity complementing antibody, A1S9T antibody, A1ST antibody, GXP 1 antibody, rabbit anti-UBE1
- 纯化方法
- The product was protein A purified from monospecific antiserum followed by further purification to remove the GST tag.
- 免疫原
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Immunogen: Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product UBA1 Primary Antibody
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anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 300-600) antibody
UBA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) antibodyUBA1 适用: 人, 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 1-1058) antibodyUBA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B6 unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 730-881) antibodyUBA1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) antibodyUBA1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 961-1058) antibodyUBA1 适用: 人, 大鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (N-Term) antibodyUBA1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (N-Term) antibodyUBA1 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (N-Term) antibodyUBA1 适用: 人, 大鼠, 小鼠, 犬, Cow, 马, 兔, 豚鼠, Bat, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 800-850) antibodyUBA1 适用: 人 IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 2 mg/mL - 20 μg/mL
Application Note: This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~118 kDa in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 5.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))
- 别名
- UBA1 (UBA1 产品)
- 背景
- Background: Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 Molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.
- 基因ID
- 7317, 23510338
- UniProt
- P22314
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