NRF1 抗体 (AA 1-534)
(3 references)
Quick Overview for NRF1 抗体 (AA 1-534) (ABIN129526)
抗原
See all NRF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-534
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Supplier Product No.
- 200-401-869
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Supplier
- Rockland
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原理
- NRF1 Antibody
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交叉反应 （详细）
- This protein A purified antibody is directed against mouse NRF1.
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产品特性
- Synonyms: rabbit anti-NRF1 antibody, rabbit anti-NRF 1 antibody, NRF-1, alpha pal antibody, alpha palindromic binding protein antibody, locus control region factor 1 antibody, NFE2 related factor 1 antibody, nuclear respiratory factor 1 antibody, transcription factor 11 antibody
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纯化方法
- The product was purified from monospecific antiserum by protein A affinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse NRF1 protein corresponding to aa 1- 534 of the native protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Nuclear Respiratory Factor 1 (NRF1) (AA 201-285) antibody
NRF1 适用: 人 WB, ELISA, IHC (p), RNAi 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) antibodyNRF1 适用: 人 WB, IHC (p), IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (AA 272-288), (Middle Region) antibodyNRF1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (Middle Region) antibodyNRF1 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, 豚鼠, 斑马鱼, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) antibodyNRF1 适用: 人 WB, IHC, IP, ChIP 宿主: 兔 Monoclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (N-Term) antibodyNRF1 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, 豚鼠, 斑马鱼, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (AA 51-180) antibodyNRF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (AA 360-409) antibodyNRF1 适用: 人, 大鼠, Cow, 兔, 马, 豚鼠, 斑马鱼, 犬, 猴, 小鸡, Pig, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (Internal Region) antibodyNRF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (AA 1-300) antibodyNRF1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 2 mg/mL - 5 μg/mL
Application Note: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to NRF1 by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:3,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.3 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Exercise Training Prevents Doxorubicin-induced Mitochondrial Dysfunction of the Liver." in: Medicine and science in sports and exercise, Vol. 51, Issue 6, pp. 1106-1115, (2019) (PubMed).
: "Mitochondrial-Targeted Antioxidant Maintains Blood Flow, Mitochondrial Function, and Redox Balance in Old Mice Following Prolonged Limb Ischemia." in: International journal of molecular sciences, Vol. 18, Issue 9, (2018) (PubMed).
: "Mitochondrial adaptations evoked with exercise are associated with a reduction in age-induced testicular atrophy in Fischer-344 rats." in: Biogerontology, Vol. 15, Issue 5, pp. 517-534, (2015) (PubMed).
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: "Exercise Training Prevents Doxorubicin-induced Mitochondrial Dysfunction of the Liver." in: Medicine and science in sports and exercise, Vol. 51, Issue 6, pp. 1106-1115, (2019) (PubMed).
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- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
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别名
- Nrf1
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背景
- Background: NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 forms a homodimer and binds DNA as a dimer. NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues. Phosphorylation of NRF1 enhances DNA binding. Multiple splice variants have been identified for this protein.
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基因ID
- 18181, 13529317
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UniProt
- Q9WU00
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
-
