(1 reference)-
- 抗原
- BUBR1 Kinase Kinase
- 抗原表位
- AA 1-350
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunoprecipitation (IP), Western Blotting (WB)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
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This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.
Immunogentype:Recombinant - 克隆位点
- 8G1
- 亚型
- IgG
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- 应用备注
- This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1% BSA.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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-
: "Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum." in: Molecular biology of the cell, Vol. 26, Issue 7, pp. 1286-95, (2016) (PubMed).
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: "Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum." in: Molecular biology of the cell, Vol. 26, Issue 7, pp. 1286-95, (2016) (PubMed).
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- 抗原
- BUBR1 Kinase Kinase
- 背景
-
BUBR1 Kinase (also called Mitotic checkpoint serine/threonine-protein kinase, BUB1 beta, MAD3/BUB1-related protein kinase, Mitotic checkpoint kinase MAD3L and SSK1) is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F, CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, it can be localized to nuclear kinetochores. This protein is highly expressed in thymus followed by spleen. Defects in BUB1B are associated with tumor formation.
Synonyms: Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody, Bub1A antibody - 基因ID
- 701
- UniProt
- O60566
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