BUBR1 Kinase Kinase (AA 1-350) 抗体

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.

Immunogen Type: Recombinant Protein

Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein.  The use of HeLa whole cell lysates is recommended as a positive control.  For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at pH 6.8.  Permeabilize fixed cells with 0.2 % Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1 % BSA.

Western Blot Dilution: 1:200 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: 1:200 - 1:1,000

Other: User Optimized

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None