BUBR1 Kinase Kinase (AA 1-350) 抗体
(1 reference)
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- 抗原
- BUBR1 Kinase Kinase
- 抗原表位
- AA 1-350
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- ELISA, Fluorescence Microscopy (FM), Immunoprecipitation (IP), Western Blotting (WB)
- Supplier Product No.
- 200-301-902
- Supplier
- Rockland
- 原理
- BUBR1 Kinase Antibody
- 交叉反应 （详细）
- This Protein A purified antibody is directed against human BUBR1 Kinase protein.
- 产品特性
- Synonyms: mouse anti-BUBR1 antibody, Beta homolog of S. cerevisiae budding uninhibited by benzimidazoles antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody, Bub1A antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Mitotic checkpoint kinase MAD3L, MAD3/BUB1-related protein kinase, hBUBR1
- 纯化方法
- The product was purified from tissue culture supernatant by chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.
Immunogen Type: Recombinant Protein
- 克隆位点
- 8G1
- 亚型
- IgG1
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- 应用备注
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Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2 % Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1 % BSA.
Western Blot Dilution: 1:200 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
IF Microscopy Dilution: 1:200 - 1:1,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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-
: "Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum." in: Molecular biology of the cell, Vol. 26, Issue 7, pp. 1286-95, (2016) (PubMed).
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: "Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum." in: Molecular biology of the cell, Vol. 26, Issue 7, pp. 1286-95, (2016) (PubMed).
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- 抗原
- BUBR1 Kinase Kinase
- 背景
- Background: BUBR1 Kinase (BUB1-related protein kinase) is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F, CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, it can be localized to nuclear kinetochores. This protein is highly expressed in thymus followed by spleen. Defects in BUB1B are associated with tumor formation.
- 基因ID
- 701
- NCBI登录号
- NP_001202
- UniProt
- O60566
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