CENPE 抗体
Quick Overview for CENPE 抗体 (ABIN129515)
抗原
See all CENPE 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-901
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Supplier
- Rockland
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原理
- CENP-E
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交叉反应 （详细）
- This Protein A purified antibody is directed against human CENP-E protein.
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产品特性
- Synonyms: mouse anti-CENP-E antibody, Centromere-associated protein E, Centromeric protein E antibody, KIF10 antibody, Kinesin-7, Kinesin family member 10 antibody, Kinesin-related protein CENPE
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纯化方法
- The product was purified from tissue culture supernatant by chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG1
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anti-Centromere Protein E, 312kDa (CENPE) antibody
CENPE 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) antibodyCENPE 适用: 人 IP, ICC, IF, FACS 宿主: 小鼠 Monoclonal 1H12 unconjugated
anti-Centromere Protein E, 312kDa (CENPE) antibodyCENPE 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (AA 2450-2590) antibodyCENPE 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (C-Term) antibodyCENPE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (C-Term) antibodyCENPE 适用: 人 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) antibodyCENPE 适用: 人 WB, IP, ICC, IF 宿主: 小鼠 Monoclonal 1H12 unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (AA 2542-2701) antibodyCENPE 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein E, 312kDa (CENPE) (AA 2222-2361) antibodyCENPE 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 320 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:200
ELISA Dilution: 1:5,000 - 1:20,000
IF Microscopy Dilution: 1:500 - 1:2,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CENPE (Centromere Protein E, 312kDa (CENPE))
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别名
- CENPE
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背景
- Background: CENP-E, Centrosome-associated protein E, is a kinesin-like minus-end directed microtubule motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENP-E is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. CENP-E interacts with CENP-F and BUBR1 kinase. CENP-E associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.
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基因ID
- 1062
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UniProt
- Q02224
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途径
- M Phase, Maintenance of Protein Location
抗原
-
