BUB1 抗体 (AA 281-419)
Quick Overview for BUB1 抗体 (AA 281-419) (ABIN129514)
抗原
See all BUB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 281-419
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Supplier Product No.
- 200-301-900
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Supplier
- Rockland
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原理
- BUB1 Antibody
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交叉反应 （详细）
- This Protein A purified antibody is directed against human BUB1 protein.
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产品特性
- Synonyms: mouse anti-BUB1 antibody, Budding uninhibited by benzimidazoles 1 homolog antibody, hBUB1 antibody, Mitotic checkpoint serine/threonine protein kinase BUB1 antibody
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纯化方法
- The product was purified from tissue culture supernatant by chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 281-419 of human BUB1 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG1
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anti-Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 1-1085) antibody
BUB1 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 1-130) antibodyBUB1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 1-1085) antibodyBUB1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4C3-4C9 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 1-130) antibodyBUB1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1A11C7 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 951-1085) antibodyBUB1 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 281-419) antibodyBUB1 适用: 人, 小鼠 WB, IF, IHC (p), ICC, FACS 宿主: 小鼠 Monoclonal 14H5 unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 1-1085) antibodyBUB1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 731-1085) antibodyBUB1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) (AA 256-577) antibodyBUB1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1) antibodyBUB1 适用: 人 WB, ELISA 宿主: 兔 Monoclonal 9C11 unconjugated Recombinant Antibody
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应用备注
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Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 160 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at pH 6.8 OR 100 % methanol at -20° C. Permeabilize fixed cells with 0.5 % Triton X-100.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:200
ELISA Dilution: 1:5,000 - 1:20,000
IF Microscopy Dilution: 1:500 - 1:2,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
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别名
- BUB1
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背景
- Background: BUB1 (also called Mitotic checkpoint serine/threonine-protein kinase BUB1and BUB1A) is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating BUB3, a member of the miotic checkpoint complex, and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. BUB1 is autophosphorylated when cells enter mitosis. This protein is localized to the nucleus in interphase cells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. BUB1 is highly expressed in testis and thymus, less in colon, spleen, lung and small intestine. Expression is associated with cells/tissues with a high mitotic index.
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基因ID
- 699
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NCBI登录号
- NP_004327
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UniProt
- O43683
抗原
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