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CDK1 抗体

Name: CDK1 抗体
SKU: ABIN129504
Availability: OutOfStock

This anti-CDK1 antibody is a 小鼠单克隆 antibody detecting CDK1 in WB, ELISA 和 IP. Suitable for 人. This Primary Antibody has been cited in 2+ publications.

Mab anti-Human p34cdc2 antibody was used to detect human p34cdc2by western blot in untreated (control) and drug treated (10 µM genistein) lysates of MCF-7 cells. (CDK1 抗体)

Mab anti-Human p34cdc2 antibody (clone POH-1) is shown to detect human p34cdc2by western blot. (CDK1 抗体)

Western Blot of Mouse anti-CDC2 (p34) antibody. (CDK1 抗体)

3 images

(2 references)

产品编号 ABIN129504

Supplier Product No.: 200-301-160

发货至: 中国

Datasheet as PDF

Quick Overview for CDK1 抗体 (ABIN129504)

抗原

See all CDK1 抗体

CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This CDK1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunoprecipitation (IP)

克隆位点

POH-1

Discover our top product CDK1 Primary Antibody

Supplier Product No.

200-301-160

Supplier

Rockland

原理

Cdc2 p34 Antibody

交叉反应（详细）

This antibody is not cross reactive with other cyclin dependent kinases.

产品特性

Synonyms: mouse anti-Cdc2 antibody, mouse anti-p34 antibody, Cell Division Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, CDC28A, CDKN1, P34CDC2

纯化方法

This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines.

过滤

Sterile filtered

免疫原

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
Immunogen Type: Recombinant Protein

亚型

IgG2a kappa
anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 59-182) antibody
CDK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (pTyr15) antibody
CDK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 211-297) antibody
CDK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G10 unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) antibody
CDK1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A4-1A9 unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 208-258) antibody
CDK1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) antibody
CDK1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 211-297) antibody
CDK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 8F1 unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) antibody
CDK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 66-297) antibody
CDK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 201-297) antibody
CDK1 适用: 人, 小鼠, 大鼠, 犬, 绵羊 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Application Note: Anti-p34 cdc2 has been tested by western blot and is suitable for immunohistochemistry, immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1 % saponin treatment or other permeabilization method.
Western Blot Dilution: 1:500 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Ye, Fincher, Tang, McNeal, Gygax, Wexler, Ryan, James, Osmani: "Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2." in: The Journal of biological chemistry, Vol. 272, Issue 52, pp. 33384-93, (1998) (PubMed).

Palmer, Gavin, Nebreda: "A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1." in: The EMBO journal, Vol. 17, Issue 17, pp. 5037-47, (1998) (PubMed).
抗原

CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

别名

CDK1

背景

Background: p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF). The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues. In humans the phosphorylation appears to be performed by p60src. The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins. The phosphorylation activity is coupled to the entry into the M-phase of the cell. p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally. Association with deletion mutants of cyclin halts the M-phase before it is completed.

基因ID

983

NCBI登录号

NP_001307847

UniProt

P06493

途径

Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA