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MAPK8 / MAPK9 (pThr183), (pTyr185) 抗体

This 兔 多克隆 antibody specifically detects MAPK8 / MAPK9 in WB, IHC (p), IF 和 IP. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN127216
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for MAPK8 / MAPK9 (pThr183), (pTyr185) 抗体 (ABIN127216)

抗原

MAPK8 / MAPK9

适用

人, 小鼠, 大鼠

宿主

  • 3

克隆类型

  • 3
多克隆

标记

  • 3
非结合性

应用范围

  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP)
  • 抗原表位

    pThr183, pTyr185

    纯化方法

    Affinity Chromatography.

    免疫原

    Synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of Human SAPK/JNK
  • 应用备注

    Western Blot: 1-2 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunocytochemistry: 10-20 μg/mL. Immunohistochemistry: 20-30 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    PBS, pH 7.2, containing 50 % Glycerol, 1 % BSA and 0.02 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C/-80 °C

    储存方法

    Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
  • 抗原

    MAPK8 / MAPK9

    背景

    The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines (such as TNF-alpha and IL-1), UV light, inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 (also known as MKK4), which in turn activates SAPK (also known as JNK). JNK binds tightly to the N-terminal region of c-Jun and ATF-2, and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.Synonyms: JNK-1, JNK-2, JNK-46, JNK-55, JNK1, JNK2, MAPK-8, MAPK-9, MAPK8, MAPK9, Mitogen-activated protein kinase 8/9, PRKM8, PRKM9, SAPK, Stress-activated protein kinase JNK11/JNK2, c-Jun N-terminal kinase 1/2
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