MAPK8 / MAPK9 (pThr183), (pTyr185) 抗体
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Quick Overview for MAPK8 / MAPK9 (pThr183), (pTyr185) 抗体 (ABIN127216)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr183, pTyr185
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纯化方法
- Affinity Chromatography.
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免疫原
- Synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of Human SAPK/JNK
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应用备注
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Western Blot: 1-2 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunocytochemistry: 10-20 μg/mL. Immunohistochemistry: 20-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- PBS, pH 7.2, containing 50 % Glycerol, 1 % BSA and 0.02 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C/-80 °C
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储存方法
- Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
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- MAPK8 / MAPK9
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背景
- The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines (such as TNF-alpha and IL-1), UV light, inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 (also known as MKK4), which in turn activates SAPK (also known as JNK). JNK binds tightly to the N-terminal region of c-Jun and ATF-2, and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.Synonyms: JNK-1, JNK-2, JNK-46, JNK-55, JNK1, JNK2, MAPK-8, MAPK-9, MAPK8, MAPK9, Mitogen-activated protein kinase 8/9, PRKM8, PRKM9, SAPK, Stress-activated protein kinase JNK11/JNK2, c-Jun N-terminal kinase 1/2
抗原
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