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NSF 抗体

This anti-NSF antibody is a 兔 多克隆 antibody detecting NSF in WB, IP 和 IHC (p). Suitable for 大鼠 和 小鼠.
产品编号 ABIN127184
发货至: 中国

Quick Overview for NSF 抗体 (ABIN127184)

抗原

See all NSF 抗体
NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

适用

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大鼠, 小鼠

宿主

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克隆类型

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多克隆

标记

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This NSF antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    The antibody recognizes 85 kDa NSF.

    纯化方法

    Protein A chromatography

    免疫原

    Synthetic peptide corresponding to amino acids around 731 of rat NSF
  • 应用备注

    Western Blot: 1-2 μg/mL. Immunoprecipitation: 5-15 mg/mL. Immunohistochemistry: 10-20 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    PBS containing 50 % glycerol, 1 % BSA, and 0.02 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.
  • 抗原

    NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

    别名

    NSF

    背景

    Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.Synonyms: N-ethylmaleimide-sensitive fusion protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF

    基因ID

    60355

    NCBI登录号

    NP_068516

    UniProt

    Q9QUL6
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