NSF 抗体
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北京 101111
Quick Overview for NSF 抗体 (ABIN127184)
抗原
See all NSF 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antibody recognizes 85 kDa NSF.
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纯化方法
- Protein A chromatography
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免疫原
- Synthetic peptide corresponding to amino acids around 731 of rat NSF
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应用备注
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Western Blot: 1-2 μg/mL. Immunoprecipitation: 5-15 mg/mL. Immunohistochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- PBS containing 50 % glycerol, 1 % BSA, and 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.
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- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
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别名
- NSF
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背景
- Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.Synonyms: N-ethylmaleimide-sensitive fusion protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF
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基因ID
- 60355
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NCBI登录号
- NP_068516
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UniProt
- Q9QUL6
抗原
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