Western Blot: 1-2 μg/mL. Immunoprecipitation: 5-15 mg/mL. Immunohistochemistry: 10-20 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.
抗原
NSF
(N-Ethylmaleimide-Sensitive Factor (NSF))
别名
NSF
背景
Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.Synonyms: N-ethylmaleimide-sensitive fusion protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF