VASP 抗体 (pSer239)
Quick Overview for VASP 抗体 (pSer239) (ABIN126912)
抗原
See all VASP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer239
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特异性
- AM00156PU-N recognizes VASP only, when Ser 239 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, mab AM00156PU-N (clone 16C2) is able to monitor the phosphorylation state of VASP serine 239 as well as PKA activity.
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产品特性
- Synonyms: Vasodilator-stimulated phosphoprotein
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纯化方法
- Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
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免疫原
- Synthetic phosphopeptide conjugated to KLHEpitope: Phosphoserine 239 RKVpSKQE
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亚型
- IgG1
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anti-Vasodilator-Stimulated phosphoprotein (VASP) antibody
VASP 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-100) antibodyVASP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibodyVASP 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibodyVASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibodyVASP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibodyVASP 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibodyVASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer238) antibodyVASP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibodyVASP 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 78-114), (N-Term) antibodyVASP 适用: 人, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA (0.05 μg/mL). Immunoblotting (0.5 μg/mL for HRPO/ECL detection). Recommended blocking buffer: BSA/Tween 20 based blocking and blot incubation buffer. Flow Cytometry. Immunoprecipitation (1-10 μg per 10^6 pervanadate-treated A431 cells). Immunocytochemistry (1-10 μg/mL, may tolerate 0.5 % formaldehyde fixation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 2x PBS containing 0.09 % Sodium Azide, PEG and Sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquot and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles!
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有效期
- 12 months
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- VASP (Vasodilator-Stimulated phosphoprotein (VASP))
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别名
- VASP
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背景
- VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines, paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.Synonyms: Vasodilator-stimulated phosphoprotein
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基因ID
- 7408
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UniProt
- P50552
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途径
- TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation
抗原
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