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VASP 抗体 (pSer239)

Name: VASP 抗体 (pSer239)
SKU: ABIN126912
Availability: OutOfStock

The 小鼠单克隆 anti-VASP antibody (Clone 16C2) (ABIN126912) specifically detects VASP in WB, IF, IP, FACS 和 EIA. The antibody is reactive with 人和小鼠 samples.

产品编号 ABIN126912

发货至: 中国

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中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for VASP 抗体 (pSer239) (ABIN126912)

抗原

See all VASP 抗体

VASP (Vasodilator-Stimulated phosphoprotein (VASP))

适用

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This VASP antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

克隆位点

16C2

Discover our top product VASP Primary Antibody

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pSer239

特异性

AM00156PU-N recognizes VASP only, when Ser 239 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, mab AM00156PU-N (clone 16C2) is able to monitor the phosphorylation state of VASP serine 239 as well as PKA activity.

产品特性

Synonyms: Vasodilator-stimulated phosphoprotein

纯化方法

Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

免疫原

Synthetic phosphopeptide conjugated to KLHEpitope: Phosphoserine 239 RKVpSKQE

亚型

IgG1
anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-100) antibody
VASP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody
VASP 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) antibody
VASP 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibody
VASP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody
VASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibody
VASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) antibody
KD Validated VASP 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB4715 unconjugated Recombinant Antibody

anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibody
VASP 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibody
VASP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibody
VASP 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA (0.05 μg/mL). Immunoblotting (0.5 μg/mL for HRPO/ECL detection). Recommended blocking buffer: BSA/Tween 20 based blocking and blot incubation buffer. Flow Cytometry. Immunoprecipitation (1-10 μg per 10^6 pervanadate-treated A431 cells). Immunocytochemistry (1-10 μg/mL, may tolerate 0.5 % formaldehyde fixation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
溶解方式

Restore with 1 mL H2O (15 min, RT).

缓冲液

2x PBS containing 0.09 % Sodium Azide, PEG and Sucrose.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquot and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles!

有效期

12 months
抗原

VASP (Vasodilator-Stimulated phosphoprotein (VASP))

别名

VASP

背景

VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines, paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.Synonyms: Vasodilator-stimulated phosphoprotein

基因ID

7408

UniProt

P50552

途径

TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation