VASP 抗体 (pSer239) (Biotin)
Quick Overview for VASP 抗体 (pSer239) (Biotin) (ABIN126909)
抗原
See all VASP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer239
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特异性
- This antibody recognizes VASP only, when Ser 239 is phosphorylated, a site preferred by cGMP-dependent protein kinase (PKG) but also used by cAMP-dependent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, antibody VASP-16C2 is able to monitor the phosphorylation state of VASP serine 239 as well as PKA activity.
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产品特性
- Synonyms: Vasodilator-stimulated phosphoprotein
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纯化方法
- Size exclusion chromatography
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免疫原
- Synthetic phosphopeptide conjugated to KLH
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亚型
- IgG1
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anti-Vasodilator-Stimulated phosphoprotein (VASP) antibody
VASP 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-100) antibodyVASP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibodyVASP 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibodyVASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibodyVASP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibodyVASP 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibodyVASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer238) antibodyVASP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibodyVASP 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 78-114), (N-Term) antibodyVASP 适用: 人, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 g per 106 pervanadate-treated A431 cells. Immunocytochemistry: 1 - 10 μg/mL. Mab VASP-16C2 may tolerate 0.5 % formaldehydefixation. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- 2 x PBS with 0.09 % Sodium Azide, PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody (Aliquote and freeze in liquid nitrogen) at -20 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
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- VASP (Vasodilator-Stimulated phosphoprotein (VASP))
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别名
- VASP
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背景
- VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines, particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.Synonyms: Vasodilator-stimulated phosphoprotein
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基因ID
- 7408
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UniProt
- P50552
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途径
- TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation
抗原
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