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VASP 抗体 (pSer157)

Name: VASP 抗体 (pSer157)
SKU: ABIN126908
Availability: OutOfStock

This anti-VASP antibody is a 小鼠单克隆 antibody detecting VASP in WB, IF, IP, FACS 和 EIA. Suitable for 人和小鼠. This Primary Antibody has been cited in 1 publication.

Phosphospecificity: Whole cell extracts of control (co) or SNP treated human platelets were applied to SDS-PAGE and transferred to PVDF membranes. (VASP 抗体 (pSer157))

1 image

(1 reference)

产品编号 ABIN126908

发货至: 中国

Quick Overview for VASP 抗体 (pSer157) (ABIN126908)

抗原

See all VASP 抗体

VASP (Vasodilator-Stimulated phosphoprotein (VASP))

适用

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This VASP antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

克隆位点

5C6

Discover our top product VASP Primary Antibody

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pSer157

特异性

AM00155PU-N recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-depedent protein kinase (PKA). The antibody does not cross-react with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, AM00155PU-N (clone 5C6) is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.

产品特性

Synonyms: Vasodilator-stimulated phosphoprotein

纯化方法

Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

免疫原

Synthetic phosphopeptide conjugated to KLHEpitope: Phosphoserine 157 RRVpSNAG

亚型

IgG1
anti-Vasodilator-Stimulated phosphoprotein (VASP) antibody
VASP 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-100) antibody
VASP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody
VASP 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody
VASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibody
VASP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibody
VASP 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibody
VASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer238) antibody
VASP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibody
VASP 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 78-114), (N-Term) antibody
VASP 适用: 人, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot ( 0.5 μg/mL for HRPO/ECL detection). Recommended: Casein/Tween 20 basedblocking and blot incubation buffer. ELISA (0.05 μg/mL). Immunoprecipitation (1 - 10 μg per 10^6 pervanadate-treated A431 cells). Immunocytochemistry (1 - 10 μg/mL, may tolerate 0.5 % formaldehyde fixation). Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
溶解方式

Restore with 1 mL H2O (15 min, RT).

缓冲液

PBS with 0.09 % Sodium Azide, PEG and Sucrose

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquot and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 2-8 °C up to 3 months. Avoid repeated freeze / thaw cycles!

有效期

12 months
Iyú, Glenn, White, Fox, Heptinstall: "Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 2, pp. 416-22, (2011) (PubMed).
抗原

VASP (Vasodilator-Stimulated phosphoprotein (VASP))

别名

VASP

背景

VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling. VASP is expressed in almost all human and animal cell lines, paricularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation. Molecular Weight: 46/50 kDaSynonyms: Vasodilator-stimulated phosphoprotein

基因ID

7408

UniProt

P50552

途径

TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation