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- 抗原 See all VASP 抗体
- VASP (Vasodilator-Stimulated phosphoprotein (VASP))
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抗原表位
- pSer157
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This VASP antibody is conjugated to FITC
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS)
- 特异性
- This antibody recognizes VASP only, when Ser157 is phosphorylated, a site preferred by cAMP-dependent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, this antibody is able to monitor the phosphorylation state of VASP Serine157 as well as PKA activity.
- 产品特性
- Synonyms: Vasodilator-stimulated phosphoprotein
- 纯化方法
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
- 免疫原
- Phosphopeptide conjugated to KLH. Epitope: Phosphoserine 157AA Sequence: R-R-V-pS-N-A-G
- 克隆位点
- 5C6
- 亚型
- IgG1
- Top Product
- Discover our top product VASP Primary Antibody
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- 应用备注
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Flow Cytometry. Immunocytochemistry: 1-10 μg/mL (may tolerate 0.5 % Formaldehyde fixation). For Immunoblotting and Immunoprecipitation use ABIN126908 and ABIN126905.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- 2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
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Aliquote and freeze (in liquid nitrogen at -20 °C to -80 °C). Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 2, pp. 416-22, (2011) (PubMed).
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Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 2, pp. 416-22, (2011) (PubMed).
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- 抗原
- VASP (Vasodilator-Stimulated phosphoprotein (VASP))
- 别名
- VASP (VASP 产品)
- 背景
- VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines, particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.Synonyms: Vasodilator-stimulated phosphoprotein
- 分子量
- 46/50 kDa
- 基因ID
- 7408
- UniProt
- P50552
- 途径
- TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation
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