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VASP 抗体 (pSer157) (Biotin)

Name: VASP 抗体 (pSer157) (Biotin)
SKU: ABIN126905
Availability: OutOfStock

VASP 适用: 人, 小鼠 WB, IF, IP, FACS, EIA 宿主: 小鼠 Monoclonal 5C6 Biotin

(1 reference)

产品编号 ABIN126905

发货至: 中国

Datasheet as PDF

抗原 See all VASP 抗体

VASP (Vasodilator-Stimulated phosphoprotein (VASP))
抗原表位
18

17

15

10

9

8

6

5

4

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer157
适用
134

64

58

11

10

9

5

2

2

2

1

1
人, 小鼠
宿主
115

18

2
小鼠
Compatible Secondaries
克隆类型
111

24
单克隆
标记
78

9

6

5

5

5

5

5

5

3

2

2

1

1

1

1

1
This VASP antibody is conjugated to Biotin
应用范围
102

51

48

25

22

14

14

14

11

11

5

4

4

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

特异性

This antibody recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-dependent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, antibody VASP-5C6 is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.

产品特性

Synonyms: Vasodilator-stimulated phosphoprotein

纯化方法

Size exclusion chromatography

免疫原

Synthetic phosphopeptide conjugated to KLH

克隆位点

5C6

亚型

IgG1

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anti-Vasodilator-Stimulated phosphoprotein (VASP) antibody
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anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-100) antibody
VASP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody
VASP 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody
VASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibody
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anti-Vasodilator-Stimulated phosphoprotein (VASP) (AA 1-380) antibody
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anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer239) antibody
VASP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) antibody
VASP 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Vasodilator-Stimulated phosphoprotein (VASP) (pSer157) antibody
VASP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 μg per 10^6 pervanadate-treated A431 cells. Immunocytochemistry: 1 - 10 μg/mL, Mab VASP-5C6 may tolerate 0.5 % formaldehydefixation. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

PBS with 0.09 % Sodium Azide, PEG and Sucrose

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store the antibody (Aliquote and freeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

有效期

12 months
Iyú, Glenn, White, Fox, Heptinstall: "Adenosine derived from ADP can contribute to inhibition of platelet aggregation in the presence of a P2Y12 antagonist." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 2, pp. 416-22, (2011) (PubMed).
抗原

VASP (Vasodilator-Stimulated phosphoprotein (VASP))

别名

VASP (VASP 产品)

别名

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背景

VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines, particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filamentSynonyms: Vasodilator-stimulated phosphoprotein

基因ID

7408

UniProt

P50552

途径

TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation