Phosphothreonine 抗体 (phosphorylated)
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北京 101111
Quick Overview for Phosphothreonine 抗体 (phosphorylated) (ABIN126868)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- phosphorylated
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纯化方法
- Size Exclusion Chromatography.
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组件
- incl. positive Control
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免疫原
- Synthetic phosphopeptide conjugated to KLH.
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亚型
- IgM
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anti-Phosphothreonine antibody
WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphothreonine antibody (FITC)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphothreonine antibody (Biotin)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphothreonine antibody (Atto 488)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphothreonine antibody (Atto 594)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 594
anti-Phosphothreonine antibody (Atto 390)WB, IP, ELISA, ICC, IF 宿主: 兔 Polyclonal Atto 390
anti-Phosphothreonine antibody (HRP)WB, IP, ELISA, IHC 宿主: 兔 Polyclonal HRP
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应用备注
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Immunoblotting (Western Blot): 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 basedblocking buffer. DO NOT USE MILK OR CASEIN FOR BLOCKING! ELISA: 0.05 μg/mL. Immunoprecipitation: 1-10 μg per 10^6 pervanadate-treated A431 cells. Positive Control: Phosphoserine/Phosphothreonine.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Positive Control: pSer / pThr Molecular Weight MarkerFormulation: The pSer/pThr molecular weight marker contains rabbit musclephosphoproteins isolated by Fe3+/IDA - affinity chromatography. Proteins are lyophilizedfrom PBS/NaF/PEG/Sucrose/ Bromophenolblue and Na - azide. After reconstitution thesolution contains 0. 09% Na-azide. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The pSer/pThr molecular weight marker is recommended for immunoblotapplications. Use 20µl molecular weight marker per lane. Note: Use BSA based blotincubation buffers. Milk, Casein and Blotto might interfere with antibody - antigeninteraction. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch.
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限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 1 week.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Phosphothreonine
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物质类
- Amino Acid
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背景
- Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.
抗原
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