Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1 mL H2O (15 min, RT).
缓冲液
1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C/-80 °C
储存方法
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Shelf life: one year from despatch.
有效期
12 months
抗原
GST
(Glutathione S Transferase (GST))
别名
Glutathione-S-Transferase (GST)
背景
The glutathione-S-transferase (GST) of Schistosoma japonicum is widely used as fusion partner in protein expression systems. The GST can be used for affinity purification of fusion proteins on immobilized glutathion as well as tag sequence if antibodies specific for the expressed protein are not available.Synonyms: GST26-Tag, Glutathione S-transferase Tag