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N-Acetylglucosamine 抗体

This 小鼠 单克隆 antibody specifically detects N-Acetylglucosamine in WB 和 EIA. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN126802
发货至: 中国
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Quick Overview for N-Acetylglucosamine 抗体 (ABIN126802)

抗原

N-Acetylglucosamine

适用

人, 小鼠, 大鼠

宿主

  • 2
小鼠

克隆类型

  • 2
单克隆

标记

  • 2
非结合性

应用范围

Western Blotting (WB), Enzyme Immunoassay (EIA)

克隆位点

10D8
  • 纯化方法

    Size exclusion chromatography

    免疫原

    N-Acetylglucosamine conjugated to KLH.

    亚型

    IgM
  • 应用备注

    Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 - 1.0 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore with 1 mL H2O (15 min, RT).

    缓冲液

    1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C/-80 °C

    储存方法

    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
    Shelf life: one year from despatch

    有效期

    12 months
  • 抗原

    N-Acetylglucosamine

    背景

    Many intracellular proteins contain monomeric O - linked N - acetylglucosamine residues conjugated to th hydroxyl group of serine or threonine residues. This modification can interfere with tserine and threonine phosphorylation.
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